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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
22/01/2021 |
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Actualizado : |
14/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DELPIAZZO, R.; BARCELLOS, M.; BARROS, S.; BENTANCOR, L.; FRAGA, M.; GIL, J.; IRAOLA, G.; MORSELLA, C.; PAOLICCHI, F.; PÉREZ, R.; RIET-CORREA, F.; SANGUINETTI, M.; SILVA, A.; SILVEIRA, C.S.; CALLEROS, L. |
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Afiliación : |
RAFAEL DELPIAZZO, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; MAILA BARCELLOS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; SOFÍA BARROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; LAURA BENTANCOR, Universidad de la República Oriental del Uruguay. Facultad de Medicina. Instituto de Higiene. Departamento de Bacteriología y Virología. Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE GIL, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; GREGORIO IRAOLA, Institut Pasteur de Montevideo. Laboratorio de Genómica Microbiana, Montevideo, Uruguay. / Universidad Mayor. Facultad de Ciencias. Centro de Biología Integrativa. Santiago de Chile, Chile.; CLAUDIA MORSELLA, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; FERNANDO PAOLICCHI, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; RUBEN PEREZ, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; ALFONSO SILVA, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUCÍA CALLEROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay. |
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Título : |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Veterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163 |
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DOI : |
10.1016/j.vas.2020.100163 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020.
Corresponding author: laurabet@higiene.edu.uy |
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Contenido : |
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. |
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Palabras claves : |
BOVINE GENITAL CAMPYLOBACTERIOSIS; CAMPYLOBACTER FETUS; MOLECULAR DIAGNOSIS; MOLECULAR DIAGNOSTICS; QPCR. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14934/1/Veterinary-Animal-Science-2021-100163.pdf
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Marc : |
LEADER 02681naa a2200373 a 4500
001 1061678
005 2021-04-14
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vas.2020.100163$2DOI
100 1 $aDELPIAZZO, R.
245 $aAccurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020. Corresponding author: laurabet@higiene.edu.uy
520 $aCampylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.
653 $aBOVINE GENITAL CAMPYLOBACTERIOSIS
653 $aCAMPYLOBACTER FETUS
653 $aMOLECULAR DIAGNOSIS
653 $aMOLECULAR DIAGNOSTICS
653 $aQPCR
700 1 $aBARCELLOS, M.
700 1 $aBARROS, S.
700 1 $aBENTANCOR, L.
700 1 $aFRAGA, M.
700 1 $aGIL, J.
700 1 $aIRAOLA, G.
700 1 $aMORSELLA, C.
700 1 $aPAOLICCHI, F.
700 1 $aPÉREZ, R.
700 1 $aRIET-CORREA, F.
700 1 $aSANGUINETTI, M.
700 1 $aSILVA, A.
700 1 $aSILVEIRA, C.S.
700 1 $aCALLEROS, L.
773 $tVeterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163
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Registro original : |
INIA Treinta y Tres (TT) |
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
31/01/2020 |
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Actualizado : |
31/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BAETHGEN, W.E.; CHRISTIANSON, C.B.; GARCIA, A. |
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Afiliación : |
WALTER E. BAETHGEN, Research and Development Division, International Fertilizer Development Center, Muscle Shoals, AL, United States; BRUCE C. CHRISTIANSON, Research and Development Division, International Fertilizer Development Center, Muscle Shoals, AL, United States; ADRIANA GARCIA LAMOTHE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Nitrogen fertilizer effects on growth, grain yield, and yield components of malting barley. |
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Fecha de publicación : |
1995 |
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Fuente / Imprenta : |
Field Crops Research, October 1995, Volume 43, Issue 2-3, Pages 87-99. |
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ISSN : |
0378-4290 |
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DOI : |
10.1016/0378-4290(95)00034-N |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 January 1994 / Accepted 6 June 1995. |
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Contenido : |
ABSTRACT.
Optimal strategies for using nitrogen fertilizer with malting barley (Hordeum vulgare L.) must aim to maximize yield while maintaining low N content in grain to preserve malting quality. Eleven field experiments were conducted during 1989-1991 with the objective of determining the impact of N fertilizer applied at different growth stages on crop growth, grain yield, and yield components of malting barley. Treatments included single applications of N fertilizer at sowing, at Zadoks growth stages Z-22 (midtillering) or Z-30 (end of tillering) and differing levels of N applied at each of these times. High rates of N applied early in the growing season stimulated tillering but many of these tillers did not produce spikes. An effect of N on spike number was evident only in comparisons of fertilized and unfertilized treatments through a positive correlation between number of spikes at maturity and the number of tillers at Z-30. Nitrogen fertilizer applied at Z-30 resulted in 30% to 100% increases in number of kernels/spike over unfertilized plots though number of kernels/spike was negatively correlated with number of spikes/m2, indicating compensation effects. Nitrogen applications at Z-30 were also most effective for increasing number of kernels/m2 while kernel weight responded only slightly to N. Mean kernel weight for all site-years revealed that none of the three years presented limiting conditions for grain filling. Cumulative probability curves for yields and yield components indicated that N fertilizer applied at Z-30 gave the best results in most situations but only when sufficient N was available at sowing to ensure crop establishment and initial tiller development. In contrast, the Z-30 treatment had the least impact on yield and yield components when no fertilizer was applied at sowing. Number of kernels/spike and number of kernels/m2 were the only two yield components with a clear association with grain yields. The research indicates that N fertilizer strategies for malting barley should ensure relatively small amounts of available N at sowing for crop establishment and initial tiller development. Additional N would then be applied at the end of tillering, the amount required depending on the crop and soil management systems used. © 1995. MenosABSTRACT.
Optimal strategies for using nitrogen fertilizer with malting barley (Hordeum vulgare L.) must aim to maximize yield while maintaining low N content in grain to preserve malting quality. Eleven field experiments were conducted during 1989-1991 with the objective of determining the impact of N fertilizer applied at different growth stages on crop growth, grain yield, and yield components of malting barley. Treatments included single applications of N fertilizer at sowing, at Zadoks growth stages Z-22 (midtillering) or Z-30 (end of tillering) and differing levels of N applied at each of these times. High rates of N applied early in the growing season stimulated tillering but many of these tillers did not produce spikes. An effect of N on spike number was evident only in comparisons of fertilized and unfertilized treatments through a positive correlation between number of spikes at maturity and the number of tillers at Z-30. Nitrogen fertilizer applied at Z-30 resulted in 30% to 100% increases in number of kernels/spike over unfertilized plots though number of kernels/spike was negatively correlated with number of spikes/m2, indicating compensation effects. Nitrogen applications at Z-30 were also most effective for increasing number of kernels/m2 while kernel weight responded only slightly to N. Mean kernel weight for all site-years revealed that none of the three years presented limiting conditions for grain filling. Cumulative probability curves for yields and yield... Presentar Todo |
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Palabras claves : |
Fertilizer; Malting barley; Nitrogen; Yield components. |
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Thesagro : |
HORDEUM VULGARE. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
https://www.sciencedirect.com/science/article/pii/037842909500034N/pdf
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Marc : |
LEADER 03072naa a2200241 a 4500
001 1060732
005 2020-01-31
008 1995 bl uuuu u00u1 u #d
022 $a0378-4290
024 7 $a10.1016/0378-4290(95)00034-N$2DOI
100 1 $aBAETHGEN, W.E.
245 $aNitrogen fertilizer effects on growth, grain yield, and yield components of malting barley.$h[electronic resource]
260 $c1995
500 $aArticle history: Received 28 January 1994 / Accepted 6 June 1995.
520 $aABSTRACT. Optimal strategies for using nitrogen fertilizer with malting barley (Hordeum vulgare L.) must aim to maximize yield while maintaining low N content in grain to preserve malting quality. Eleven field experiments were conducted during 1989-1991 with the objective of determining the impact of N fertilizer applied at different growth stages on crop growth, grain yield, and yield components of malting barley. Treatments included single applications of N fertilizer at sowing, at Zadoks growth stages Z-22 (midtillering) or Z-30 (end of tillering) and differing levels of N applied at each of these times. High rates of N applied early in the growing season stimulated tillering but many of these tillers did not produce spikes. An effect of N on spike number was evident only in comparisons of fertilized and unfertilized treatments through a positive correlation between number of spikes at maturity and the number of tillers at Z-30. Nitrogen fertilizer applied at Z-30 resulted in 30% to 100% increases in number of kernels/spike over unfertilized plots though number of kernels/spike was negatively correlated with number of spikes/m2, indicating compensation effects. Nitrogen applications at Z-30 were also most effective for increasing number of kernels/m2 while kernel weight responded only slightly to N. Mean kernel weight for all site-years revealed that none of the three years presented limiting conditions for grain filling. Cumulative probability curves for yields and yield components indicated that N fertilizer applied at Z-30 gave the best results in most situations but only when sufficient N was available at sowing to ensure crop establishment and initial tiller development. In contrast, the Z-30 treatment had the least impact on yield and yield components when no fertilizer was applied at sowing. Number of kernels/spike and number of kernels/m2 were the only two yield components with a clear association with grain yields. The research indicates that N fertilizer strategies for malting barley should ensure relatively small amounts of available N at sowing for crop establishment and initial tiller development. Additional N would then be applied at the end of tillering, the amount required depending on the crop and soil management systems used. © 1995.
650 $aHORDEUM VULGARE
653 $aFertilizer
653 $aMalting barley
653 $aNitrogen
653 $aYield components
700 1 $aCHRISTIANSON, C.B.
700 1 $aGARCIA, A.
773 $tField Crops Research, October 1995, Volume 43, Issue 2-3, Pages 87-99.
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